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Sunday, May 18, 2008

Examples of Industrial Enzymes

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Enzymes Source or Type Applications
Carbohydrases   Laundry and dishwashing detergents, industrial pipe/tank cleaners, textiles, pulp and paper, fermentation ethanol
Alpha-amylase Bacterial a-amylase (e.g., Bacillus subtilis), Fungal a-amylase (e.g., Aspergillus niger), Alkaline a-amylase Textiles, starch syrups, laundry and dishwashing detergents, paper desizing, fermentation ethanol, animal feed
ß-amylase From a strain of Bacillus Brewing, maltose syrup
Cellulase   Dishwashing detergents, animal feed, textiles, bioenergy production
ß-Glucanase exo-ß-1,4-glucanase, endo-ß-1,4-glucanase Brewing industry
ß-Glucosidase   Transforms isoflavone phytoestrogens in soymilk
Dextranase Made by various microorganisms (e.g., Leuconostoc mesenteriodes) Hydrolyzes the polysaccharide dextran
Dextrinase (almost identical to a-glucosidase)   Cleaves dextrin into two molecules of glucose
a-Galactosidase (melibiase)   Could increase yield of sucrose; potential use in the beet sugar industry
Glucoamylase Aspergillus niger, Rhizopus, Endomyces Manufacture of dextrose syrup and high-fructose syrup
Hemmicellulase/Pentosanase/Xylanase Thermomyces lanuginosus, Penicillium simplicissimum Baking, fruit juice manufacture, wood pulp processing
Invertase   Manufacture of invert syrup from cane or beet sugar (use is minor)
Lactase Kluyveromyces lactis, Asperigillus oryzae, Bacillus Eliminates lactose from dairy foods
Naringinase   Debitter citrus peel
Pectinase   Fruit processing
Pullulanase Klebsiella aerogenes, Bacillus acidipullulyticus, Bacillus subtilis Antistaling agent in baked goods
Proteases   Brewing, baking goods, protein processing, distilled spirits, laundry and dishwashing detergents, lens cleaners, leather and fur, chemicals
Acid proteinase Endothia parasitica, Rhizopus, Aspergillus niger, A. oryzae Baking, improves dough handling
Alkaline protease Bacillus subtilis, Bacillus licheniformis Detergents, leather and fur
Bromelain Pineapple stem Food industry
Pepsin Porcine or bovine stomach Cheese production
Peptidases    
Aminopeptidase Lactococcus lactis Food and animal feed
Endo-peptidase    
Subtilisin Bacillus subtilis var. Carlsberg, Bacillus lichenformis Chiral resolution of chemical compounds or pharmaceuticals
Lipases and Esterases Phospholidases, pregastric esterases, phosphatases Cleaners, leather and fur, dairy, chemicals
Aminoacylase Porcine kidney, Aspergillus melleus Optical resolution of amino acids
Glutaminase Bacillus, Aspergillus Conversion of glutamine to glutamate
Lysozyme Chicken egg white, Saccharomyces cerevisiae, Pichia pastoris Antibacterial (germicidal in dairy industry)
Penicillin acylase Bacillus megaterium, Escherichia coli Chemical synthesis
Isomerase   Converts glucose syrup to high-fructose syrup in food industry
Oxireductases   Chemicals, detergent bleaches, pulp bleaching
Alcohol dehydrogenase Saccharomyces cerevisiae, Thermoanarobium brockii Chiral synthesis of chemicals
Amino acid oxidase Porcine kidney, snake venom Chiral resolution of racemic amino acid mixtures
Catalase Aspergillus niger desugaring of eggs
Chloroperoxidase Algae, bacteria, fungi, mammalian tissues Steroid synthesis
Peroxidase Horseradish Laundry and wood pulp bleaches
Lyases    
Acetolactate decarboxylase   Brewing industry
Aspartic ß-decarboxylase   Manufacture of L-alanine from L-aspartic acid
Histidase Achromobacter liquidum Cosmetics
Transferases    
Cyclodextrin glycosyltransferase   Manufacture of cyclodextrins from starch

Source: Diversa & Novo Nordisk

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